Some characteristics of lower molecular weight arbovirus RNA's
نویسندگان
چکیده
منابع مشابه
The presence of high-molecular-weight viral RNAs interferes with the detection of viral small RNAs.
Viral small interfering RNA (siRNA) accumulation in plants is reported to exhibit a strong strand polarity bias, with plus (+) strand siRNAs dominating over minus (-) strand populations. This is of particular interest, as siRNAs processed from double-stranded RNA would be expected to accumulate equivalent amounts of both species. Here, we show that, as reported, (-) strand viral siRNAs are dete...
متن کاملComplementarity of sequences in low molecular weight RNAs to regions of messenger and ribosomal RNAs.
Total low molecular weight nuclear RNAs of mouse ascites cells have been labeled in vitro and used as probes to search for complementary sequences contained in nuclear or cytoplasmic RNA. From a subset of hybridizing lmw RNAs, two major species of 58,000 and 35,000 mol. wt. have been identified as mouse 5 and 5.8S ribosomal RNA. Mouse 5 and 5.8S rRNA hybridize not only to 18 and 28S rRNA, respe...
متن کاملHigh molecular weight RNAs and small interfering RNAs induce systemic posttranscriptional gene silencing in plants.
Posttranscriptional gene silencing (PTGS) in transgenic plants is an epigenetic form of RNA degradation related to PTGS and RNA interference (RNAi) in fungi and animals. Evidence suggests that transgene loci and RNA viruses can generate double-stranded RNAs similar in sequence to the transcribed region of target genes, which then undergo endonucleolytic cleavage to generate small interfering RN...
متن کاملMolecular and biological characteristics of some geographic isolates of nucleopolyhedrovirus of Helicoverpa armigera (Lep.: Noctuidae)
هفت جدایه از ویروس Helicoverpa armigera NPV با تکنیک PCR-RFLP توصیف گردید و بیماری‌زایی نسبی آنها نسبت به لاروهای سن دوم H. armigera (Hübner) قبل و بعد از پرتوتابی با شبه نور خورشید و شرایط محیط آزاد روی دو گیاه پنبه و گوجه‌فرنگی مورد مقایسه قرار گرفت. محصول PCR حاصل از ژن 25K FP تفاوتی بین جدایه‌ها نشان نداد (780 جفت باز). محصول پلیمراز به دست آمده از جدایه‌ها پس از آنالیز جداگانه با چهار نوع ...
متن کاملSynthesis of Some High-molecular Weight Esters in Supercritical Alcohols
Esterification of several carboxylic acids with supercritical alcohols such as 1-propanol, 1butanol, 1-octanol, isoamylalcohol, ethanol, methanol, and subcritical benzyl alcohol without using any catalyst was studied. A variety of esters were synthesized very effectively using supercritical alcohols as solvent and reagent. Moderate to excellent yields were obtained depending on the nature of th...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 1972
ISSN: 0014-5793
DOI: 10.1016/0014-5793(72)80559-3